Confocal microscopy provides optical sectioning, the ability to observe discrete planes in 3D samples, by using one or more apertures to block out-of-focus light. Nikon offers both point-scanning confocal instruments, led by our AX / AX R confocal system, as well as spinning disk (field scanning) confocal systems from top manufacturers.
Multiphoton microscopy is preferred for deep imaging applications in thick specimens, including intravital imaging. Non-linear excitation restricts fluorescence to the laser focus and near-infrared illumination minimizes absorption and scattering. Nikon offers the AX R MP multiphoton system, available with microscope stand options optimized for large specimens.
Image scanning microscopy (ISM) is a super-resolution technique that takes advantage of structured detection of each point in a point-scanning system to improve both resolution and signal-to-noise (S/N), a great choice for low light imaging. Both the AX / AX R confocal and AX R MP multiphoton systems may be equipped with the Nikon Spatial Array Confocal (NSPARC) detector for ISM imaging.
Nikon AX /AX R with NSSPARC Confocal Microscope
Improving on Perfection.
Confocal microscopes have been commercially available now for over 25 years. How can newer iterations of a fundamentally simple instrument continue to innovate? What changes can redefine how a confocal is used, and what data can be collected? Introducing the Nikon AX/AX R Confocal Microscope System, our 10th generation point scanning confocal, giving you more of everything: Leveraging Artificial Intelligence (AI), expanding the number of colors, improving pixel density, sensitivity and speed.
These are significant additions in terms of expanding the range of experiments possible with a point scanning confocal, while increasing the usability and functionality of the instrument, all in a modular and upgradable platform.
Nikon AX is the new standard in confocal imaging.
Nikon Crest X-Light Spinning Disk Confocal Microscope
Spinning disk confocal solutions for challenging research applications
The X-light confocal series, fully integrated with Nikon’s superb microscope platforms and optics, is designed to cover any imaging need: from a budget-friendly upgrade of a widefield microscope into a confocal system, to a cutting-edge confocal system for the most challenging applications.
The strength of CrestOptics solutions relies on the high quality of the optical design and on the flexibility of each component, allowing to generate tailor-made solutions to match even the most demanding challenges.
X-light confocal series: See Brighter, See Faster, See More